PMID:16299298

Citation	Leatham, MP, Stevenson, SJ, Gauger, EJ, Krogfelt, KA, Lins, JJ, Haddock, TL, Autieri, SM, Conway, T and Cohen, PS (2005) Mouse intestine selects nonmotile flhDC mutants of Escherichia coli MG1655 with increased colonizing ability and better utilization of carbon sources.Infect. Immun. 73:8039-49
Abstract	D-gluconate which is primarily catabolized via the Entner-Doudoroff (ED) pathway, has been implicated as being important for colonization of the streptomycin-treated mouse large intestine by Escherichia coli MG1655, a human commensal strain. In the present study, we report that an MG1655 Deltaedd mutant defective in the ED pathway grows poorly not only on gluconate as a sole carbon source but on a number of other sugars previously implicated as being important for colonization, including L-fucose, D-gluconate, D-glucuronate, N-acetyl-D-glucosamine, D-mannose, and D-ribose. Furthermore, we show that the mouse intestine selects mutants of MG1655 Deltaedd and wild-type MG1655 that have improved mouse intestine-colonizing ability and grow 15 to 30% faster on the aforementioned sugars. The mutants of MG1655 Deltaedd and wild-type MG1655 selected by the intestine are shown to be nonmotile and to have deletions in the flhDC operon, which encodes the master regulator of flagellar biosynthesis. Finally, we show that DeltaflhDC mutants of wild-type MG1655 and MG1655 Deltaedd constructed in the laboratory act identically to those selected by the intestine; i.e., they grow better than their respective parents on sugars as sole carbon sources and are better colonizers of the mouse intestine.
Links	PubMed Online version:10.1128/IAI.73.12.8039-8049.2005
Keywords	Animals; Carbohydrate Metabolism; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Gene Deletion; Genes, Bacterial; Gluconates; Intestines; Mice; Trans-Activators
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: edd Genotype of Experimental Strain : edd(del)	Reference Condition:					Mutations were non-motile after growth in LB and failed to spread on agar, figure 7.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: flhD Genotype of Experimental Strain : flhD(del)	Reference Condition:					Mutations were non-motile after growth in LB and failed to spread on agar, figure 7.
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