PMID:1648558

Citation	Greenberg, JT, Chou, JH, Monach, PA and Demple, B (1991) Activation of oxidative stress genes by mutations at the soxQ/cfxB/marA locus of Escherichia coli.J. Bacteriol. 173:4433-9
Abstract	Exposure of Escherichia coli to superoxide-generating drugs, such as menadione or paraquat, uniquely induces approximately 40 proteins, nine of which are under the positive control of the soxR locus (at min 92). We report here that certain mutations at a separate locus that we have named soxQ (at min 34) confer some of the phenotypes seen in soxR-constitutive strains, including resistance to menadione. A previously known mutation called cfxB, identified through antibiotic resistance, is likely an allele of soxQ. The soxQ1 and cfxB mutations cause transcriptional activation of the genes that encode Mn-containing superoxide dismutase, glucose 6-phosphate dehydrogenase, and the soi-17/19::lac and soi-28::lac fusions. These genes are also activated by soxR, but the soxQ1 and cfxB mutations increase the synthesis of seven other proteins not influenced by soxR. Moreover, the soxQ1- and cfxB-dependent phenotypes do not depend on the soxR gene, and gene induction by soxR in response to redox stress does not depend on the soxQ locus. As well as increasing cellular resistance to some oxidants, the soxQ1 and cfxB mutations confer elevated resistance to various antibiotics, probably via diminished expression of outer membrane protein OmpF. The marA1 multiple-antibiotic resistance mutation (also at min 34) behaves like a weak allele of soxQ but probably resides in a nearby gene that, with soxQ, is part of a regulatory complex. We propose that soxQ helps control some oxidative stress proteins as part of another regulon that responds to an unknown environmental signal.
Links	PubMed
Keywords	Anti-Bacterial Agents; Crosses, Genetic; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genotype; Glucosephosphate Dehydrogenase; Mutagenesis; Phenotype; Superoxide Dismutase; Transcriptional Activation; Transduction, Genetic; Vitamin K
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JHC1113 NCBI Taxon ID: 83333	Genotype of Reference Strain: marA Genotype of Experimental Strain : marA1	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to Menadione, table 2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: cxfB Genotype of Experimental Strain : cfxB1	Reference Condition:	OMP:0000276	increased resistance to antimicrobial compound			provided increased resistance to ampicillin and tetracycline and constitutively over-expressed the micF RNA
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: soxQ Genotype of Experimental Strain : soxQ1	Reference Condition:	OMP:0000276	increased resistance to antimicrobial compound			expresses multiple antibiotic resistance (elevated resistance to redox agents- menadione- and antibiotics- chloramphenicol and nalidixic acid) and diminished rate of synthesis of OmpF. also showed increased resistance to ampicillin and tetracycline Constitutively over-expressed the micF RNA
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