PMID:1650909

Citation	Itaya, M and Crouch, RJ (1991) Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H. Mol. Gen. Genet. 227:433-7
Abstract	The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnhA+ dnaA+ Genotype of Experimental Strain : rnhA-1 dnaA508	Reference Condition: 32oC Experimental Condition: 42oC			ECO:0000178	in vivo assay evidence	The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnhA+ dnaA+ Genotype of Experimental Strain : rnhA-59 dnaA508	Reference Condition: 32oC Experimental Condition: 42oC			ECO:0000178	in vivo assay evidence	The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnhA+ dnaA+ Genotype of Experimental Strain : rnhA-91(amber) dnaA508	Reference Condition: 32oC Experimental Condition: 42oC			ECO:0000178	in vivo assay evidence	The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnhA+ dnaA+ Genotype of Experimental Strain : rnhA-224 (UGA) dnaA508	Reference Condition: 32oC Experimental Condition: 42oC			ECO:0000178	in vivo assay evidence	The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1157 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnhA+ dnaA+ Genotype of Experimental Strain : rnhA-339::cat dnaA508	Reference Condition: 32oC Experimental Condition: 42oC			ECO:0000178	in vivo assay evidence	The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MIC1002 NCBI Taxon ID: 83333	Genotype of Reference Strain: rnh+ recB+ Genotype of Experimental Strain : rnh-1 recB270	Reference Condition: 32oC Experimental Condition: 43oC			ECO:0000178	in vivo assay evidence	The rnh mutation caused a decrease in RNase H activity. See Table S3.
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