PMID:1697149
| Citation |
Sizemore, RK , Caldwell, JJ and Kendrick, AS (1990) Alternate gram staining technique using a fluorescent lectin. Appl. Environ. Microbiol. 56:2245-7 |
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| Abstract |
Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed. |
| Links | |
| Keywords |
Acetylglucosamine/metabolism; Bacteria/classification; Bacteria/metabolism; Cell Membrane/metabolism; Fluorescent Dyes; Gentian Violet; Phenazines; Staining and Labeling/methods; Wheat Germ Agglutinins |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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