PMID:1703938

Citation	Van Horn, KG and Dworkin, BM Direct gram stain and urease test to detect Helicobacter pylori. Diagn. Microbiol. Infect. Dis. 13:449-52
Abstract	Antral biopsies were obtained by gastrointestinal endoscopy on 143 adult patients with dyspeptic symptoms of gastritis or peptic ulcer disease. A direct Gram stain and a direct urease test were performed on each biopsy in addition to culture. Forty-three biopsies (30%) were considered positive for Helicobacter pylori based on culture or histologic examination, or both. Thirty-one biopsies (72% sensitivity) were positive for both direct tests, whereas 95 of 100 negative cultures were negative for both tests. Thirty-eight of the 43 positive biopsies were Gram stain positive (sensitivity, 88%; specificity, 100%). The direct urease test alone was positive at 4 hr for 29 biopsies (sensitivity, 67%; specificity, 100%) and at 24 hr for 38 biopsies (sensitivity, 74%; specificity, 95%). Rapid presumptive diagnosis of H. pylori in antral biopsies was obtained when at least one direct test, Gram stain or urease, was positive.
Links	PubMed
Keywords	Adult; Biopsy; Gastritis/microbiology; Gastroscopy; Gentian Violet; Helicobacter Infections/microbiology; Helicobacter pylori/isolation & purification; Humans; Peptic Ulcer/microbiology; Phenazines; Predictive Value of Tests; Pyloric Antrum; Staining and Labeling; Stomach/microbiology; Urease
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
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