PMID:1721886
| Citation |
Williams, MD, Fuchs, JA and Flickinger, MC (1991) Null mutation in the stringent starvation protein of Escherichia coli disrupts lytic development of bacteriophage P1.Gene 109:21-30 |
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| Abstract |
As initial steps toward understanding the regulation and function of the stringent starvation protein (SSP) of Escherichia coli, we have isolated the ssp gene (encoding SSP), defined the operon in which ssp is found, and created insertion-deletion mutations of the ssp gene in recBC, sbc and recD strains by linear DNA transformation. During attempts to move the insertion-deletion structure to other strains by P1 transduction, we found that P1 was unable to form plaques on hosts lacking an intact ssp gene. The delta ssp mutation, however, did not affect transduction of the delta ssp strains and mutant strains were able to support lysogenic P1. When P1 lytic growth was induced, an increase in P1 DNA was detected without lysis or plaque formation. Examination of proteins synthesized in the delta ssp host during induction revealed the absence of P1 late gene products. Also, the apparent continued synthesis of early gene products during late time points was observed in the delta ssp host. The results reported here suggest that the defect in P1 lytic growth brought about by the absence of SSP occurs at the point at which bacteriophage P1 shifts from early to late gene expression. We also report the results of experiments on stable RNA synthesis following amino acid (aa) starvation induced by serine hydroxamate, and experiments on stable RNA synthesis following resupplementation of a limiting aa. These experiments show that SSP is not involved in stable RNA synthesis. Additionally, complementation studies have shown that ssp is identical to the previously described pog gene of E. coli. |
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| Keywords |
Bacterial Proteins; Base Sequence; Cell Cycle; Cell Death; Cloning, Molecular; Coliphages; DNA Mutational Analysis; Escherichia coli; Gene Expression Regulation, Bacterial; Lysogeny; Molecular Sequence Data; Operon; RNA; Transcription, Genetic; Transduction, Genetic |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to antibiotics |
0000144 |
Southwestern analysis |
sspA was removed conferring Neomycin resistance. Analyzed by southern blot. |
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Notes
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