PMID:17379776
| Citation |
Ishii, N , Nakahigashi, K , Baba, T , Robert, M , Soga, T , Kanai, A , Hirasawa, T , Naba, M , Hirai, K , Hoque, A , Ho, PY , Kakazu, Y , Sugawara, K , Igarashi, S , Harada, S , Masuda, T , Sugiyama, N , Togashi, T , Hasegawa, M , Takai, Y , Yugi, K , Arakawa, K , Iwata, N , Toya, Y , Nakayama, Y , Nishioka, T , Shimizu, K , Mori, H and Tomita, M (2007) Multiple high-throughput analyses monitor the response of E. coli to perturbations. Science 316:593-7 |
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| Abstract |
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. |
| Links |
PubMed Online version:10.1126/science.1132067 |
| Keywords |
Chromatography, Liquid; Computational Biology; Electrophoresis, Capillary; Electrophoresis, Gel, Two-Dimensional; Enzyme Induction; Enzyme Repression; Enzymes/genetics; Enzymes/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression; Genes, Bacterial; Mass Spectrometry; Metabolic Networks and Pathways/genetics; Mutation; Oligonucleotide Array Sequence Analysis; Proteome; RNA, Messenger/genetics; RNA, Messenger/metabolism; Systems Biology/methods; Transcription, Genetic |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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