PMID:18627634
Citation |
Flaberg, E , Sabelström, P , Strandh, C and Szekely, L (2008) Extended Field Laser Confocal Microscopy (EFLCM): combining automated Gigapixel image capture with in silico virtual microscopy. BMC Med Imaging 8:13 |
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Abstract |
Confocal laser scanning microscopy has revolutionized cell biology. However, the technique has major limitations in speed and sensitivity due to the fact that a single laser beam scans the sample, allowing only a few microseconds signal collection for each pixel. This limitation has been overcome by the introduction of parallel beam illumination techniques in combination with cold CCD camera based image capture. |
Links |
PubMed PMC2515298 Online version:10.1186/1471-2342-8-13 |
Keywords |
Animals; Artificial Intelligence; Equipment Design; Equipment Failure Analysis; Image Enhancement/instrumentation; Image Interpretation, Computer-Assisted/instrumentation; Microscopy, Confocal/instrumentation; Pattern Recognition, Automated/methods; Phantoms, Imaging; Rats; Reproducibility of Results; Sensitivity and Specificity; Signal Processing, Computer-Assisted/instrumentation; User-Computer Interface |
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