PMID:1925019

Citation	Trun, NJ and Gottesman, S Characterization of Escherichia coli mutants with altered ploidy.Res. Microbiol. 142:195-200
Abstract	We describe the isolation and characterization of new mutants in the cell cycle of Escherichia coli. The mutants were selected as gain of function mutants that are able to maintain more than the normal number of chromosomes. Our increased ploidy mutants were isolated as resistant to camphor vapours, which selects for cells with more chromosomes than normal. The mutants (called mbr for moth-ball-resistant) map to four chromosomal locations: mbrA at 68 min; mbrB at 88.5 min; mbrC at 89.5 min; and mbrD at 90 min. To investigate the nature of these cell cycle mutants, we have coupled them with defects in recA, to test for induction of the SOS response, and dam, to determine if methylation is required for mbr function. Based on the results of these and other tests, we have made a preliminary placement of the mbr mutants within the context of the cell cycle. mbrA mutations appear to be defective in the coupling of the DNA replication cycle to the cell division cycle, and as such, may define a new link between the two processes. mbrB does not seem to be able to coordinate the cell cycle and the growth rate of the cell. mbrC appears to be defective in partitioning of chromosomes. mbrD, which may be allelic to rpoB (a subunit of RNA polymerase), appears to be defective in either chromosomal partitioning or the later stages of DNA replication.
Links	PubMed
Keywords	Camphor; Cell Cycle; Cell Division; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Genes, Dominant; Mutation; Ploidies; Replicon
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mrbA Genotype of Experimental Strain : mrbA4	Reference Condition:	OMP:0000276	increased resistance to antibiotics			camphor resistance
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: mrbA Genotype of Experimental Strain : mrbA15	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Resistant to camphor
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 8333	Genotype of Reference Strain: mbrC Genotype of Experimental Strain : mbrC11	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Camphor Resistance
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 8333	Genotype of Reference Strain: mbrD Genotype of Experimental Strain : mbrD3	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Camphor Resistance, Several other similar phenotype mutants are described
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 8333	Genotype of Reference Strain: mbrD Genotype of Experimental Strain : mbrD7	Reference Condition:	OMP:0000276	increased resistance to antibiotics			Resistance to camphor, other mutants with similar phenotypes are described
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