PMID:20519959
| Citation |
Spirek, M, Benko, Z, Carnecka, M, Rumpf, C, Cipak, L, Batova, M, Marova, I, Nam, M, Kim, DU, Park, HO, Hayles, J, Hoe, KL, Nurse, P and Gregan, J (2010) S. pombe genome deletion project: an update. Cell Cycle 9:2399-402 |
|---|---|
| Abstract |
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames. |
| Links |
PubMed PMC3132452 Online version:10.4161/cc.9.12.11914 |
| Keywords |
DNA Restriction Enzymes; Gene Deletion; Gene Knockout Techniques/methods; Genetic Vectors; Genome, Fungal; Open Reading Frames; Plasmids; Polymerase Chain Reaction; Schizosaccharomyces/genetics |
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
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