PMID:21183718
| Citation |
Washburn, RS and Gottesman, ME (2011) Transcription termination maintains chromosome integrity.Proc. Natl. Acad. Sci. U.S.A. 108:792-7 |
|---|---|
| Abstract |
DNA replication fork movement is impeded by collisions with transcription elongation complexes (TEC). We propose that a critical function of transcription termination factors is to prevent TEC from blocking DNA replication and inducing replication fork arrest, one consequence of which is DNA double-strand breaks. We show that inhibition of Rho-dependent transcription termination by bicyclomycin in Escherichia coli induced double-strand breaks. Cells deleted for Rho-cofactors nusA and nusG were hypersensitive to bicyclomycin, and had extensive chromosome fragmentation even in the absence of the drug. An RNA polymerase mutation that destabilizes TEC (rpoB*35) increased bicyclomycin resistance >40-fold. Double-strand break formation depended on DNA replication, and can be explained by replication fork collapse. Deleting recombination genes required for replication fork repair (recB and ruvC) increased sensitivity to bicyclomycin, as did loss of the replication fork reloading helicases rep and priA. We propose that Rho responds to a translocating replisome by releasing obstructing TEC. |
| Links |
PubMed Online version:10.1073/pnas.1009564108 |
| Keywords |
Bicyclo Compounds, Heterocyclic; Chromosomes; DNA Mutational Analysis; DNA Replication; DNA-Directed DNA Polymerase; DNA-Directed RNA Polymerases; Escherichia coli; Escherichia coli Proteins; Models, Genetic; Mutation; Peptide Elongation Factors; Transcription Factors; Transcription, Genetic; Ultraviolet Rays |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
