PMID:21216997
| Citation |
Hale, CA, Shiomi, D, Liu, B, Bernhardt, TG, Margolin, W, Niki, H and de Boer, PA (2011) Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers.J. Bacteriol. 193:1393-404 |
|---|---|
| Abstract |
Assembly of the cell division apparatus in bacteria starts with formation of the Z ring on the cytoplasmic face of the membrane. This process involves the accumulation of FtsZ polymers at midcell and their interaction with several FtsZ-binding proteins that collectively organize the polymers into a membrane-associated ring-like configuration. Three such proteins, FtsA, ZipA, and ZapA, have previously been identified in Escherichia coli. FtsA and ZipA are essential membrane-associated division proteins that help connect FtsZ polymers with the inner membrane. ZapA is a cytoplasmic protein that is not required for the fission process per se but contributes to its efficiency, likely by promoting lateral interactions between FtsZ protofilaments. We report the identification of YcbW (ZapC) as a fourth FtsZ-binding component of the Z ring in E. coli. Binding of ZapC promotes lateral interactions between FtsZ polymers and suppresses FtsZ GTPase activity. This and additional evidence indicate that, like ZapA, ZapC is a nonessential Z-ring component that contributes to the efficiency of the division process by stabilizing the polymeric form of FtsZ. |
| Links |
PubMed Online version:10.1128/JB.01245-10 |
| Keywords |
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Main Points of the Paper
- zapC is determined to be another member of the septal ring
- ZapC suppresses FtsZ GTPase activity
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
BL5 |
zapC-Slm260 |
longer cell length in late-logrithmic growth |
Morphology |
when grown to late log phase, this mutant appears slightly longer on average than those of the wild-type parent, TB28 |
Microscopy |
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|
Escherichia coli |
BL5 |
del minCDE zapC-Slm260 |
longer cell length in late-logrithmic growth |
Morphology |
when grown to late log phase, this mutant appears slightly longer on average than those of the wild-type parent, TB43 |
Microscopy |
||
|
Escherichia coli |
BL6 |
del minCDE zapC-Slm260 |
increased length-to-septum ratio |
Morphology |
increased from 17 micrometers in TB43 to 55 micrometers in BL6 |
Microscopy |
Table 3 | |
|
Escherichia coli |
BL6 |
del minCDE zapC-Slm260 |
filamentous |
Morphology |
suppressed by ectopic expression of zapC |
Microscopy |
Figure 2 | |
| edit table |
</protect>
Notes
References
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