PMID:2838458
| Citation |
No author listed (1988) Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods. J. Bacteriol. 170:3094-101 |
|---|---|
| Abstract |
The Escherichia coli minB mutant originally isolated is known to septate at cell poles to form spherical anucleate minicells. Three new minicell-producing mutants were isolated during a screening by autoradiography for chromosome partition mutants giving rise spontaneously to normal-sized anucleate cells. These min mutants were affected close to or in the minB locus. Autoradiography analysis as well as fluorescent staining of DNA showed that in addition to minicells, these strains and the original minB mutant also spontaneously produced anucleate rods of normal size and had an abnormal DNA distribution in filaments. These aberrations were not associated with spontaneous induction of the SOS response. Inhibition of DNA synthesis in these mutants gave rise to anucleate cells whose size was longer than unit cell length, suggesting that the min defect allows septation to take place at normally forbidden sites not only at cell poles but also far from poles. Abnormal DNA distribution and production of anucleate rods suggest that the Min product(s) could be involved in DNA distribution. |
| Links | |
| Keywords |
Autoradiography; Cell Division; Cell Nucleus; Cyclic AMP/metabolism; DNA, Bacterial/analysis; DNA, Bacterial/biosynthesis; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/ultrastructure; Fluorescent Dyes; Indoles; Microscopy, Fluorescence; Mutation; Receptors, Cyclic AMP/metabolism; SOS Response (Genetics) |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
