PMID:2847279

Citation	Bouloc, P, Jaffé, A and D'Ari, R Preliminary physiologic characterization and genetic analysis of a new Escherichia coli mutant, lov, resistant to mecillinam.Rev. Infect. Dis. 10:905-10
Abstract	A mutant of Escherichia coli resistant to mecillinam was selected. The target of this antibiotic is penicillin-binding protein 2 (PBP2), which is active in cell wall elongation and responsible for the rod-like shape of the organism. Several observations indicate that PBP2 remains active in the mutant. For example, cells are small and rod-shaped in the absence of mecillinam but become spherical in its presence; the formation of filaments in the presence of furazlocillin shows that cell wall elongation still takes place. The mutant also has a low growth rate and small cell volume, even in rich medium. In these respects, the mutant resembles cya or crp strains (in which the cyclic adenosine monophosphate-catabolite activator protein [cAMP-CAP] complex is inactive), although its catabolic capacities are unaffected. The mutation, called lov, lies at 41 minutes on the E. coli genetic map. The phenotype is partially suppressed by the P1-mediated transduction of an rpsL ribosomal mutation conferring streptomycin resistance.
Links	PubMed
Keywords	Amdinocillin; Bacterial Proteins; Carrier Proteins; Culture Media; Cyclic AMP; Escherichia coli; Hexosyltransferases; Multienzyme Complexes; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin Resistance; Penicillin-Binding Proteins; Peptidyl Transferases; Phenotype; Transduction, Genetic
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GC2700 NCBI Taxon ID: 83333	Genotype of Reference Strain: lov Genotype of Experimental Strain : lov in strain GC2702	Reference Condition:	0000276	increased resistance to antimicrobial compound			Resistance to Mecillinam (A B Lactam).
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GC3700 NCBI Taxon ID: 83333	Genotype of Reference Strain: argS Genotype of Experimental Strain : argS201	Reference Condition:	0000276	increased resistance to antimicrobial compound			Resistance to Mecillinam (A B Lactam).
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GC3702 NCBI Taxon ID: 83333	Genotype of Reference Strain: alaS Genotype of Experimental Strain : alaS21	Reference Condition:	0000276	increased resistance to antimicrobial compound			Resistance to Mecillinam (A B Lactam).
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Notes

OMP ID: Mecillinam is a penicillin Derivative. Waiting on OMP term to approved.

ECOID: Plate Assay.

References

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