PMID:2953652

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Citation

Odoevskaia, ER and Sineokiĭ, SP (1987) [Isolation and genetic analysis of bacterial mutations gpr blocking the replication of various lambda phages]. Genetika 23:643-52

Abstract

Bacterial mutations affecting phi 80 DNA replication have been isolated and designated gpr2, 27. The main difference between gpr2, 27 and groP, grp mutations described earlier is that mutations gpr2, 27 are not essential for lambda replication. The mutations have been mapped between thr and leu loci in the vicinity of mutations groPC756 (dnaK gene) and groPC259 (dnaJ gene), their order being: thr-gpr2-groPC756-groPC259-gpr27. Complementation analysis using transducing phages suggested that mutations gpr2, 27 are localized in genes unknown earlier, outside dnaK and dnaJ genes.

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Keywords

Bacteriophage lambda/genetics; Bacteriophage lambda/physiology; Chromosome Mapping; Escherichia coli/genetics; Genes, Bacterial; Genetic Markers; Mutation; Transduction, Genetic; Virus Replication



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