PMID:328489
| Citation |
Bassford, PJ Jr, Diedrich, DL, Schnaitman, CL and Reeves, P (1977) Outer membrane proteins of Escherichia coli. VI. Protein alteration in bacteriophage-resistant mutants. J. Bacteriol. 131:608-22 |
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| Abstract |
Protein 1 was shown to be the receptor for phage PA-2 by the observations that the purified protein inactivates the phage, mutants lacking the protein are resistant to the phage, and mutants selected for PA-2 resistance have altered protein. Protein 1 appears as two bands (1a and 1b) on high-resolution polyacrylamide gels. The most abundant classes of mutants (ParI and ParII) selected for PA-2 resistance were found to lack band 1b. The mutations responsible for the ParI and ParII phenotypes were mapped at a locus termed par, which is near nalA on the Escherichia coli chromosome. The cyanogen bromide peptides of proteins 1a and 1b are similar, suggesting that these bands represent modified forms of the same polypeptide. Strains carrying the tolF mutation produce only band 1b. When a par tolF double mutant was constructed, this strain produced only band 1a. These results suggest that genes at the par and tolF loci are involved in modification of protein 1, or regulation of such modification, and are not structural genes for protein 1. |
| Links | |
| Keywords |
Bacterial Proteins/analysis; Bacterial Proteins/biosynthesis; Bacterial Proteins/physiology; Chromosome Mapping; Chromosomes, Bacterial; Coliphages; Escherichia coli/metabolism; Escherichia coli/physiology; Genes; Lysogeny; Membrane Proteins/analysis; Membrane Proteins/biosynthesis; Membrane Proteins/physiology; Mutation |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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