PMID:334721

Citation	Reiner, AM (1977) Xylitol and D-arabitol toxicities due to derepressed fructose, galactitol, and sorbitol phosphotransferases of Escherichia coli.J. Bacteriol. 132:166-73
Abstract	d-Arabitol was observed to be toxic to many laboratory strains of Escherichia coli K-12, and xylitol was found to be toxic to an existing E. coli C mutant strain. Fructose-specific components of the phosphoenolpyruvate:sugar phosphotransferase system are required for xylitol toxicity. Selection for xylitol resistance results in Fru(-) strains blocked in fructose phosphotransferase. Introduction of the ptsF or ptsI mutation into a xylitol-sensitive strain eliminates sensitivity. [(14)C]fructose uptake experiments imply that the mutation to xylitol sensitivity, which is co-transducible with ara and leu, results in derepression of normally inducible fructose phosphotransferase. Wild-type strains also become xylitol sensitive if induced by (and then removed from) fructose. Xylitol toxicity is prevented by fructose in both wild-type and mutant strains. Circumstances causing xylitol, a new food additive, to become toxic to an otherwise insensitive wild-type organism have not been reported previously. The d-arabitol-sensitive laboratory strains are galactitol (dulcitol) utilizers, although most other strains are not. Selection for d-arabitol resistance results in Gat(-) strains blocked in a constitutive galactitol-specific component of the phosphotransferase system. A mutation causing d-arabitol sensitivity occurred many years ago in AB284, the parent of AB311, AB312, AB313, and many other strains. d-Arabitol sensitivity also occurs in sorbitol-constitutive strains and is shown, like the previous two instances of pentitol toxicities, to result from a constitutive phosphotransferase, which is blocked in mutants selected for resistance.
Links	PubMed
Keywords	Arabinose; Enzyme Repression; Escherichia coli; Fructose; Galactitol; Genes; Mutation; Phenotype; Phosphotransferases; Sorbitol; Sugar Alcohols; Xylitol
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: 1278 NCBI Taxon ID: 83333	Genotype of Reference Strain: srlA+ Genotype of Experimental Strain : srlA in mutant strain	Reference Condition:	0000284	loss of utilization of carbon-containing compound	0000181	in vitro assay data	Ribotol dehydrogenase constitutive but unable to utilize xylitol for growth.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 1278S NCBI Taxon ID: 83333	Genotype of Reference Strain: srlA+ Genotype of Experimental Strain : srlA 1278S ptsF	Reference Condition:	0000266	utilizes carbon-containing compound	0000181	in vitro assay data	Introduction of ptsF mutations causes xylitol tolerance, see table 2 for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 1278S NCBI Taxon ID: 83333	Genotype of Reference Strain: srlA+ Genotype of Experimental Strain : srlA+ 1278S ptsI	Reference Condition:	0000266	utilizes carbon-containing compound	0000181	in vitro assay data	Introduction of ptsI mutation causes xylitol tolerance and utilization.
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