PMID:342516

Citation	Long, WS, Slayman, CL and Low, KB (1978) Production of giant cells of Escherichia coli.J. Bacteriol. 133:995-1007
Abstract	Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells.
Links	PubMed
Keywords	Cell Division; Escherichia coli; Microscopy, Phase-Contrast; Mutation; Phenotype; Transduction, Genetic; Ultraviolet Rays
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: LL81 NCBI Taxon ID: 83333	Genotype of Reference Strain: ampA⁺ envB⁺ sloB⁺ lon⁺ Genotype of Experimental Strain : ampA1 envB1 sloB1 lon-10	Reference Condition:	0000041	increased cell size	0000181	in vitro assay data	The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. both strains are from previous work.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: LL81 NCBI Taxon ID: 83333	Genotype of Reference Strain: ampA⁺ envB⁺ sloB⁺ lon⁺ Genotype of Experimental Strain : ampA1 envB1 sloB1 lon-10	Reference Condition:	0000041	increased cell size	0000181	in vitro assay data	The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. Irradiation causes heterogeneity in cell shape. See figure 1 for visualization 5 hours after irradiation.
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