PMID:349355

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Citation

Chippaux, M, Giudici, D, Abou-Jaoudé, A, Casse, F and Pascal, MC (1978) Laboratoire de Chimie Bactérienne C.N.R.S., Marsielle, France. Mol. Gen. Genet. 160:225-9

Abstract

Mutants of E. coli, completely devoid of nitrite reductase activity with glucose or formate as donor were studied. Biochemical analysis indicates that they are simultaneously affected in nitrate reductase, nitrite reductase, fumarate reductase and hydrogenase activities as well as in cytochrome C552 biosynthesis. The use of an antiserum specific for nitrate reductase shows that the nitrate reductase protein is probably missing. A single mutation is responsible for this phenotype: the gene affected, nir R, is located close to tyr R i.e. at 29 min on the chromosomal map.

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Keywords

Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Fumarates; Genes; Glucose/metabolism; Mutation; NADH, NADPH Oxidoreductases/genetics; Nitrate Reductases/genetics; Nitrite Reductases/genetics; Oxidoreductases/genetics



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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status


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