PMID:3543211

Citation	Laviña, M, Pugsley, AP and Moreno, F (1986) Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12.J. Gen. Microbiol. 132:1685-93
Abstract	We have identified mutations in three different chromosomal genes of Escherichia coli K12 which reduce sensitivity to microcin B17. Mutations in ompF and ompR genes affected production of an outer membrane porin protein, OmpF, and resulted in reduced sensitivity to a number of other agents (colicins, bacteriophages) besides microcin B17. The third class of mutants were specifically and highly resistant to microcin B17. The mutations in these strains were mapped to a gene (sbmA), located at 8.7 min on the E. coli K12 chromosome, which is closely linked to phoA. The wild-type sbmA allele was cloned into multiple copy number plasmids, and its location within the cloned DNA fragment was further defined by mutagenesis with MiniMudII1681. These insertion mutations resulted in in-frame fusions between the sbmA and lacZ genes, thereby allowing us to determine the direction of sbmA gene transcription. Plasmids carrying these gene fusions produced low levels of beta-galactosidase, indicating that the sbmA gene is poorly expressed. We have been unable to identify the sbmA gene product, but indirect evidence indicates that it might be an envelope protein involved in microcin uptake.
Links	PubMed
Keywords	Anti-Bacterial Agents; Bacteriocins; Chromosome Mapping; Cloning, Molecular; Escherichia coli; Genes, Bacterial; Mutation; Phenotype; Transduction, Genetic
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC714 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbmA+ Genotype of Experimental Strain : BM21 sbmA11::Tn5	Reference Condition:	0000276	increased resistance to antibiotics	0000033	traceable author statement	Insertion of transposon into sbmA gene increased the occurrence Microcin B17 resistance. Author statement on page 1686
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC745 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbmA+ Genotype of Experimental Strain : BM11 sbmA11::Tn5	Reference Condition:	0000276	increased resistance to antibiotics	0000033	traceable author statement	Insertion of transposon into sbmA gene increased the occurrence Microcin B17 resistance and Kanamycin resistance. Author statement on page 1686
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC514 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR+ Genotype of Experimental Strain : ompR-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	The introduction ompR mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MH407 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompF+ Genotype of Experimental Strain : ompF-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	The introduction ompF mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC514 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR+ recA+ Genotype of Experimental Strain : ompR- recA-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	The introduction ompR and recA mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MH407 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompF+ recA+ Genotype of Experimental Strain : ompF- recA-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	The introduction ompF and recA mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC760 NCBI Taxon ID: 83333	Genotype of Reference Strain: recA+ Genotype of Experimental Strain : recA-	Reference Condition:	0000275	decreased resistance to antibiotics	0000181	in vitro assay data	Insertion of recA mutant increased sensitivity toward microcin B17. See table 4 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MH150 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompC+ Genotype of Experimental Strain : ompC-	Reference Condition:	0000276	Increased resistance to antibiotics	0000181	in vitro assay data	Insertion of ompC mutation causes a slight decrease in microcin B17 sensitivity. See table 4 for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC730 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbmA+ Genotype of Experimental Strain : sbma-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	Insertion of sbmA mutation caused complete resistance to microcin B17. See table 4.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RYC730 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbmA+ recA+ Genotype of Experimental Strain : sbma- recA-	Reference Condition:	0000276	increased resistance to antibiotics	0000181	in vitro assay data	Insertion of sbmA mutation caused complete resistance to microcin B17 even with the recA mutation. See table 4.
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