PMID:363701

Citation	Dabbs, ER (1978) Kasugamycin-dependent mutants of Escherichia coli.J. Bacteriol. 136:994-1001
Abstract	Kasugamycin-dependent mutants have been isolated from Escherichia coli B. They were obtained through mutagenesis with ethyl methane sulfonate or nitrosoguanidine in conjunction with an antibiotic underlay technique. In the case of nitrosoguanidine, dependent mutants were obtained at a frequency of about 3% of survivors growing up in the selection. In the case of ethyl methane sulfonate, the corresponding value was 1%. Nineteen mutants showing a kasugamycin-dependent phenotype were studied. In terms of response to various temperatures and antibiotic concentrations, they were very heterogeneous, although most fell into two general classes. Genetic analysis indicated that in at least some cases, the kasugamycin-dependent phenotype was the product of two mutations. Two-dimensional gel electropherograms revealed alterations in the ribosomal proteins of seven mutants. One mutant had an alteration in protein S13, and one had an alteration in protein L14. Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form.
Links	PubMed
Keywords	Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Dose-Response Relationship, Drug; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Temperature
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: rpsR+ Genotype of Experimental Strain : Ksg r rpsR MV9	Reference Condition:	0000276	increased resistance to antimicrobial compound	0000181	in vitro assay data	Kasugamycin resistance developed when the strain was grown 42C.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: rpsR+ Genotype of Experimental Strain : Ksg d rpsR MV9	Reference Condition:	0005101	aminoglycoside dependent growth	0000181	in vitro assay data	Kasugamycin dependence developed when the strain was grown 30C. See Figure 2b for gel electropherogram. Strains MV 2, 29, 34 and 35 had were also Ksgd, see table 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: rpsM+ Genotype of Experimental Strain : Ksg d rpsM MV34	Reference Condition:	0005101	aminoglycoside dependent growth	0000181	in vitro assay data	Kasugamycin dependence developed when the strain was grown 30C. See Figure 2E for gel electropherogram of 70S ribosome protein.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: rpsI+ Genotype of Experimental Strain : Ksgd rpsI MV9	Reference Condition:	0005101	aminoglycoside dependent growth	0000181	in vitro assay data	Kasugamycin dependence was dependent on the temperature it was grown at; 30 C. See table 1 and figure 2b. Strain MV21 had similar results see figure 2c.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: Ksgs rpsR+ L44 Genotype of Experimental Strain : Ksgd rpsR- MV3	Reference Condition:	0005101	aminoglycoside dependent growth	0000033	traceable author statement	MV3 was found to be Kasugamycin dependent as well as have growth which was independent of temperature. See page 995 for full list of similar mutant strains.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: prmA+ Genotype of Experimental Strain : Ksgd prmA MV9	Reference Condition:	0005101	aminoglycoside dependent growth	0000181	in vitro assay data	Kasugamycin dependence developed. See Figure 2 for partial to dimensional gel electropherogram of the 70S ribosomal protein.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: B Substrain: L44 NCBI Taxon ID: 37762	Genotype of Reference Strain: Ksgs rpsR+ L44 Genotype of Experimental Strain : Ksgd rpsR- MV4	Reference Condition:	0005101	aminoglycoside dependent growth	0000033	traceable author statement	MV4 was found to be Kasugamycin dependent but with growth that was temperature dependent. See page 995 for full list of similar mutant strains.
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Notes

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References

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