PMID:374348
| Citation |
Baughman, GA and Fahnestock, SR (1979) Chloramphenicol resistance mutation in Escherichia coli which maps in the major ribosomal protein gene cluster.J. Bacteriol. 137:1315-23 |
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| Abstract |
Localized mutagenesis and selection for streptomycin resistance were utilized to isolate a chloramphenicol resistance mutation in Escherichia coli K-12 linked to the strA (rpsL) locus. Bacteriophage P1 transduction verified the map position of the new resistance mutation at 72 min, placing it within a dense cluster of ribosomal protein genes. The map position differs from that of known cmlA and cmlB mutations, which map at 18 and 21 min, respectively. Ribosomes prepared from chloramphenicol-resistant and -sensitive isogenic transductants were analyzed in vitro for activity in formation of N-formylmethionyl-puromycin, polyphenylalanine, and polylysine in the presence of inhibitory concentrations of chloramphenicol. Comparisons were also made of 14C-chloramphenicol binding to 70S ribosomes and of the two-dimensional polyacrylamide gel electrophoresis pattern of ribosomal proteins from each strain. There was no detectable difference between ribosomes from sensitive and resistant strains as measured by these assays. Enzymatic modification by chloramphenicol acetyltransferase is not responsible for the observed phenotype. |
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| Keywords |
Chloramphenicol; Chromosome Mapping; Drug Resistance, Microbial; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Streptomycin; Transduction, Genetic |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to antimicrobial compound |
Resistant to choramphenicol as measured by zones of growth inhibition. |
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to antimicrobial compound |
Resistant to choramphenicol as measured by zones of growth inhibition. |
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Notes
OMP terms: Chloromphinicol has not distinct structure classification
ECOID term: Culture assay (uses culture dishes with antibiotics discs to measure growth inhibition).
References
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