PMID:387731

Citation	Anderson, JJ, Wilson, JM and Oxender, DL (1979) Defective transport and other phenotypes of a periplasmic "leaky" mutant of Escherichia coli K-12.J. Bacteriol. 140:351-8
Abstract	A mutant of Escherichia coli K-12 deficient in high-affinity leucine transport and related binding proteins was obtained by selecting for azaleucine resistance after bacteriophage Mu mutagenesis. We determined that the cause was a generalized loss of periplasmic binding proteins and a sharp decrease in the activity of transport systems requiring them. Other transport systems resistant to osmotic shock and present in membrane vesicles, were affected to a lesser degree or not at all. The mutation, designated lky::Mucts, was shown to be a pleiotropic envelope mutation, rendering the mutant sensitive to ionic and nonionic detergents, antibiotics, and ethylenediaminetetraacetic acid: the strain had also acquired tolerance to colicins E1, E2, and E3, while remaining normally sensitive to a variety of bacteriophages. An analysis of the lipopolysaccharide of parent and mutant strains revealed a twofold reduction in the neutral sugar content of the core oligosaccharide of the lky strain, but no change in sensitivities to phages which utilize lipopolysaccharide or outer membrane proteins for absorption. The lky::Mucts locus was mapped by transduction and found to be located near, or in, the tolPAB gene cluster linked to gal. Secondary mutations suppressing the detergent sensitivity of lky arose at a frequency of 10(-7), yielding a variety of new phenotypes. The lky::Mucts mutation did not give rise to obvious alterations in the gross morphology of the cell or in cell division.
Links	PubMed
Keywords	Bacterial Proteins; Biological Transport, Active; Carrier Proteins; Cell Membrane; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Genes; Mutation
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AE84 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolB+ Genotype of Experimental Strain : AE84064 'tolB::Mucts	Reference Condition:					The tolB mutation caused continuous secretion of newly synthesized periplasmic protein during growth.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AE84 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolB+ Genotype of Experimental Strain : AE84064 'tolB::Mucts	Reference Condition:					The tolB mutation confers reduced transport capacity with reduced activity in various transport systems including the Methylgalactoside system, LIV-I system, Lysine-arginine-ornithine system and glutamine transport system. Approximately 2 to 3 times reduced rate. See table 2 for full results
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AE84 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolB+ Genotype of Experimental Strain : AE84064 'tolB::Mucts	Reference Condition:					The tolB mutation conferred a weakened ability to grow in various carbons sources. While only slightly less than parent strain in rich broth (LBT) is was significantly less efficient in glucose medium.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AE84 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolB+ Genotype of Experimental Strain : AE84064 'tolB::Mucts	Reference Condition:
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