PMID:3918006
| Citation |
Sullivan, MA and Bock, RM (1985) Isolation and characterization of antisuppressor mutations in Escherichia coli.J. Bacteriol. 161:377-84 |
|---|---|
| Abstract |
Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT. |
| Links | |
| Keywords |
Base Sequence; Codon; Escherichia coli; Genes, Bacterial; Lac Operon; Mutation; Pseudouridine; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Transduction, Genetic; beta-Galactosidase |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
0000049 |
reporter gene assay |
The efficiency of suppression was measured and there was seen to be a significant decrease as seen in table 5. |
|||
|
a mutation or genetic difference within a strain |
|
|
|
0000049 |
reporter gene assay |
The efficiency of suppression was measured and there was seen to be a significant decrease as seen in table 5. |
| ||
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
