PMID:4570598
| Citation |
Willsky, GR, Bennett, RL and Malamy, MH (1973) Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation.J. Bacteriol. 113:529-39 |
|---|---|
| Abstract |
Two classes of alkaline phosphatase constitutive mutations which comprise the original phoS locus (genes phoS and phoT) on the Escherichia coli genome have been implicated in the regulation of alkaline phosphatase synthesis. When these mutations were introduced into a strain dependent on a single system, the pst system, for inorganic phosphate (P(i)) transport, profound changes in P(i) transport were observed. The phoT mutations led to a complete P(i) (-) phenotype in this background, and no activity of the pst system could be detected. The introduction of the phoS mutations changed the specificity of the pst system so that arsenate became growth inhibitory. Changes in the phosphate source led to changes in the levels of constitutive alkaline phosphatase synthesis found in phoS and phoT mutants. When glucose-6-phosphate or l-alpha-glycerophosphate was supplied as the sole source of phosphate, phoT mutants showed a 3- to 15- fold reduction in constitutive alkaline phosphatase synthesis when compared to the maximal levels found in limiting P(i) media. However, these levels were still 100 times greater than the basal level of alkaline phosphatase synthesized in wild-type strains under these conditions. The phoS mutants showed only a two- to threefold reduction when grown with organic phosphate sources. The properties of the phoT mutants selected on the basis of constitutive alkaline phosphatase synthesis were similar in many respects to those of pst mutants selected for resistance to growth inhibition caused by arsenate. It is suggested that the phoS and phoT genes are primarily involved in P(i) transport and, as a result of this function, play a role in the regulation of alkaline phosphatase synthesis. |
| Links | |
| Keywords |
Alkaline Phosphatase; Arsenic; Biological Transport, Active; Culture Media; Drug Resistance, Microbial; Enzyme Repression; Escherichia coli; Genes, Regulator; Glucose; Glucosephosphates; Glycerol; Glycerophosphates; Mutation; Phosphates; Phosphorus Isotopes; Recombination, Genetic; Spectrophotometry; Stereoisomerism; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
ECO:0000033 |
traceable author statement |
Resistant to Arsenate |
|||
|
a mutation or genetic difference within a strain |
|
|
|
ECO:0000033 |
traceable author statement |
Resistant to Arsenate |
|||
|
a mutation or genetic difference within a strain |
|
|
|
ECO:0000033 |
traceable author statement |
Resistant to Arsenate |
|||
|
a mutation or genetic difference within a strain |
|
|
|
ECO:0000033 |
traceable author statement |
Resistant to Arsenate |
| ||
| edit table |
</protect>
Notes
Arsenate compound contains arsenate. Arsenates are salts or esters of arsenic acid. and OMP is resistance to arsenate.
References
See Help:References for how to manage references in omp dev.
