PMID:4613268

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Citation

Schreckenberger, PC and Blazevic, DJ (1974) Rapid methods for biochemical testing of anaerobic bacteria. Appl Microbiol 28:759-62

Abstract

Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-beta-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for esculin and gelatin showed an agreement of 86 and 77%, respectively. PathoTec test strips for nitrate, indole, esculin, o-nitrophenyl-beta-D-galactopyranoside, Voges-Proskauer, and urease were also tested and showed encouraging results.

Links

PubMed PMC186820

Keywords

Anaerobiosis; Bacteria/classification; Bacteria/metabolism; Bacteriological Techniques; Esculin/metabolism; Evaluation Studies as Topic; Galactose; Galactosidases/metabolism; Gelatin/metabolism; Glycosides/metabolism; Hydrolysis; Indoles/biosynthesis; Nitrates/metabolism; Nitrobenzenes; Oxidation-Reduction; Reagent Strips; Starch/metabolism

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Materials and Methods Used

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