PMID:4868365
| Citation |
Kaplan, S and Anderson, D (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.J. Bacteriol. 95:991-7 |
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| Abstract |
A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed. |
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| Keywords |
Amino Acids; Bacterial Proteins; Carbon Isotopes; Escherichia coli; Ligases; Molecular Biology; Mutation; Proline; RNA, Bacterial; Temperature; Thymine; Tritium; Uracil |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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ECO:0000182 |
in vitro culture assay data |
Thymineless death resistance in thyA. Under the condition of 42C. |
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Notes
References
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