PMID:4908785

Citation	Benson, CE, Love, SH and Remy, CN (1970) Inhibition of de novo purine biosynthesis and interconversion by 6-methylpurine in Escherichia coli.J. Bacteriol. 101:872-80
Abstract	The inhibition of Escherichia coli strain B and strain W-11 by 6-methylpurine depended on the formation of 6-methylpurine ribonucleotide by the action of adenine phosphoribosyltransferase (AMP: pyrophosphate phosphoribosyltransferase, EC 2.4.2.7). 6-Methylpurine ribonucleotide inhibited the de novo synthesis of purines, presumably via pseudofeedback inhibition of phosphoribosylpyrophosphate amidotransferase (EC 2.4.2.14). The same mechanism accounted for its inhibition of adenylosuccinate synthetase [IMP: l-aspartate ligase (GDP), EC 6.3.4.4]. Adenine and 6-methylaminopurine prevented inhibition by competing for the action of adenine phosphoribosyltransferase. In addition, adenine reversed this inhibition by replenishing the AMP to bypass both sites of inhibition. Nonproliferating suspensions of strain B-94, which lacked adenylosuccinate lyase (EC 4.3.2.2), converted exogenous hypoxanthine and aspartate to succinoadenine derivatives which accumulated in the medium. Compounds which inhibited adenylosuccinate synthetase inhibited accumulation of the succinoadenine derivatives. A method was described for the isolation of mutants which potentially possessed an altered adenylosuccinate synthetase.
Links	PubMed
Keywords	Adenine; Carbon Isotopes; Chromatography, Paper; Depression, Chemical; Electrophoresis; Escherichia coli; Genetics, Microbial; Ligases; Lyases; Mutation; Purines; Spectrophotometry; Transferases; Ultraviolet Rays
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: B and W-11 NCBI Taxon ID: 83333	Genotype of Reference Strain: purA Genotype of Experimental Strain : purA	Reference Condition:					Resistance to Methyl(6)purine + hypoxanthine
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