PMID:4920090
| Citation |
Ames, GF and Lever, J (1970) Components of histidine transport: histidine-binding proteins and hisP protein.Proc. Natl. Acad. Sci. U.S.A. 66:1096-103 |
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| Abstract |
The high-affinity (K(m) = 3 x 10(-8) M) transport system for histidine in Salmonella typhimurium has been resolved into three components: J, K, and P. J, which is a histidine-binding protein released by osmotic shock, is specified by the hisJ gene: hisJ mutants lack the binding protein and are defective in histidine transport. Another class of mutants-dhuA, which is closely linked to hisJ-has five times the normal level of binding protein and has an increased rate of histidine transport. P, which is a protein specified by the hisP gene, is required for J binding protein to be operative in transport. hisP mutants, though defective in transport, have normal levels of J binding protein. K, a third transport component, works in parallel to J, and also requires the P protein in order to be operative in transport. A second histidine-binding protein has been found but its relation to K is unclear. hisJ, dhuA, and hisP have been mapped and are in a cluster (near purF) on the S. typhimurium chromosome. |
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| Keywords |
Biological Transport; Chromatography, DEAE-Cellulose; Chromosome Mapping; Histidine; Mutation; Protein Binding; Salmonella typhimurium; Tritium |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Salmonella enterica spp. Typhimurium |
TA831 |
hisF645 |
no growth on D-histidine |
Growth |
Plating Assay |
Table 1 | ||
|
Salmonella enterica spp. Typhimurium |
TA831 |
hisF645 |
|
Sensitivity to |
HIPA is an inhibitory histidine analog |
Table 1 | ||
|
Salmonella enterica spp. Typhimurium |
TA1014 |
dhuA1 |
|
Sensitivity to |
HIPA is an inhibitory histidine analog |
Table 1 | ||
|
Salmonella enterica spp. Typhimurium |
TA1653 |
hisJ5601 |
|
Metabolic Activity |
Lineweaver-Burk plot |
Biochemical Assay |
Figure 2 | |
|
Salmonella enterica spp. Typhimurium |
TA1195 |
hisP5503 |
|
Metabolic Activity |
Lineweaver-Burk plot |
Biochemical Assay |
Figure 2 | |
|
Salmonella enterica spp. Typhimurium |
hisP1661 |
|
Metabolic Activity |
Lineweaver-Burk plot |
Biochemical Assay |
Figure 2 | ||
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</protect>
Notes
References
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