PMID:4927942

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Citation

Nishimura, Y, Caro, L, Berg, CM and Hirota, Y (1971) Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.J. Mol. Biol. 55:441-56

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Integrative Suppression; Hfr; Phage; Acridine Orange; Ethidium Bromide; DNA Replication; Reversion; Temperature-sensitivity

Main Points of the Paper

  • New type of suppression of a temperature-sensitive mutation of DNA replication- the chromosome may be incorporated into the F replicon in Hfr strains

Materials and Methods Used

  • Acridine orange sensitivity assay
  • EtBr sensitivity assay
  • Cross-streak test for conjugation
  • Transduction

Phenotype Annotations

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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:83333

CRT46

  • slow stop DNA replication defect
  • abolished initiation of DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

CRT226

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

PAT42

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay


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Notes

References

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