PMID:4942764
| Citation |
Russell, RR and Pittard, AJ (1971) Mutants of Escherichia coli unable to make protein at 42 C.J. Bacteriol. 108:790-8 |
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| Abstract |
Members of a collection of mutants of Escherichia coli unable to form colonies on nutrient agar at 42 C have been characterized on the basis of their growth response to a shift from 32 to 42 C in liquid medium. Forty-four mutants, which show an abrupt, nonlethal cessation of growth when moved to the restrictive temperature, have been characterized with respect to the effect of the mutation responsible for temperature sensitivity on deoxyribonucleic acid, ribonucleic acid, and protein synthesis. In 12 mutants, the mutation causing temperature sensitivity of growth primarily affects protein synthesis, in each case through an altered aminoacyl-transfer ribonucleic acid synthetase. Mutants with temperature-sensitive glutamyl-, phenylalanyl-, and valyl-transfer ribonucleic acid synthetases have been obtained, and the genes specifying these enzymes have been mapped by conjugation and transduction. Another mutant has been shown to possess a temperature-sensitive tryptophanyl-transfer ribonucleic acid synthetase, but this is not responsible for inability to grow at 42 C on media containing tryptophan. |
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| Keywords |
Agar; Amino Acyl-tRNA Synthetases; Bacterial Proteins; Carbon Isotopes; Cell-Free System; Chromosome Mapping; Conjugation, Genetic; Culture Media; DNA, Bacterial; Escherichia coli; Genes; Glutamates; Hot Temperature; Leucine; Mutagens; Mutation; Nitrosoguanidines; Phenylalanine; RNA, Bacterial; Thymidine; Transduction, Genetic; Tritium; Uracil; Valine |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000298 |
enzyme activity phenotype |
0000181 |
in vitro assay evidence |
Mutation caused the deletion of the valS gene resulting in the absence of valy-tRNA synthetase activities at 42 C. See Table 5 for full experimental results. |
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a mutation or genetic difference within a strain |
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0000298 |
enzyme activity phenotype |
0000181 |
in vitro assay evidence |
The mutation to the pheS gene caused a strong decrease in pheS activity. This was also seen in starins 353 and 359. See table 7 for full experimental data. |
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Notes
- AB3282 & KA56 mutants could be broken up into 5 groups, labeled A through E. Group A displayed no net increase in cell density an viable count with a normal morphology at 42 C. Group B could only grow for 1 to 5 hours at 42 C before growth was abruptly stopped. In C group mutants died at 42 C or lysed at various times. Group D grew at rather reduced but still exponential rate at 42 C. Group E had a period of roughly 7 hours were growth was gradually decreased but never stopped completely, phenotypically they formed long filaments.
References
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