PMID:4958494
Citation |
Fowler, AV and Zabin, I (1966) Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains.Science 154:1027-9 |
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Abstract |
Many nonsense mutants that map in the beta-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native beta-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated. |
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Keywords |
Chromosome Mapping; Escherichia coli; Galactosidases; Genes; Immunodiffusion; Mutation; Ultracentrifugation |
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Materials and Methods Used
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Phenotype Annotations
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Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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References
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