PMID:4958494

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Citation

Fowler, AV and Zabin, I (1966) Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains.Science 154:1027-9

Abstract

Many nonsense mutants that map in the beta-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native beta-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated.

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Keywords

Chromosome Mapping; Escherichia coli; Galactosidases; Genes; Immunodiffusion; Mutation; Ultracentrifugation

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

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