PMID:6243629

Citation	Tamaki, S, Matsuzawa, H and Matsuhashi, M (1980) Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli.J. Bacteriol. 141:52-7
Abstract	Two closely linked genes, mrdA and mrdB, located at ca. 14.2 min on the Escherichia coli chromosomal linkage map, seen to be responsible for the normal rod shape and mecillinam sensitivity of E. coli. The product of mrdA was concluded to be penicillin-binding protein 2, because mrdA mutations caused formation of thermosensitive penicillin-binding protein 2. The product of the mrdB gene is unknown. At 42 degrees, C, mutation in either of these genes caused formation of spherical cells and mecillinam resistance. Both mutations was recessive, and complementation, as detected in +-/-+ meroheterodiploids having the wild-type phenotype, provided strong evidence that the two mutations are in different complementation groups. P1 transduction suggested that the most plausible gene order is leuS-mrdA-mrdB-lip. The rodA mutation reported previously seems to be similar to the mrdB murations, but the identities of the two have not yet been proven.
Links	PubMed
Keywords	Amdinocillin; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli; Genes; Genetic Linkage; Mutation; Penicillanic Acid; Penicillin Resistance
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TMM1 NCBI Taxon ID: 83333	Genotype of Reference Strain: mrdA Genotype of Experimental Strain : mrdA1	Reference Condition: 30C Experimental Condition: 42C	OMP:0000276	increased resistance to antibiotics	in vitro culture assay data	ECO:0000182	Resistance to mecillinams, strains TMM2, TMM3, TMM45 also have same phenotype.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TMM1 NCBI Taxon ID: 83333	Genotype of Reference Strain: mrdA Genotype of Experimental Strain : mrdA1	Reference Condition: 30C Experimental Condition: 42C	OMP:0000073	cell shape phenotype	ECO:0000176	mutant visible phenotype analysis	Spherical shaped cells, Strains TMM2, TMM3,TMM5 have same phenotype
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TMM4 NCBI Taxon ID: 83333	Genotype of Reference Strain: mrdB Genotype of Experimental Strain : mrdB4	Reference Condition: 30C Experimental Condition: 42C	OMP:0000276	increased resistance to antimicrobial compound	ECO:0000182	in vitro culture assay data	MEcillinam resistance, Strain TMM6 also have same phenotype.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: TMM4 NCBI Taxon ID: 83333	Genotype of Reference Strain: mrdB Genotype of Experimental Strain : mrdB4	Reference Condition:	OMP:0000073	cell shape phenotype	ECO:0000176	mutant visible phenotype analysis	Spherical shaped cells, strain TMM6 also had the same phenotype.
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