PMID:6247616

Citation	Danchin, A and Dondon, L (1980) Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate.Mol. Gen. Genet. 178:155-64
Abstract	E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity.
Links	PubMed
Keywords	Butyrates; Butyric Acids; Culture Media; Cyclic AMP; Drug Resistance, Microbial; Escherichia coli; Guanosine Pentaphosphate; Guanosine Tetraphosphate; Keto Acids; Mutation; Phenotype; Serine
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: FB8 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbA+ Genotype of Experimental Strain : SDT1 sbaA-	Reference Condition: 33oC Experimental Condition: 43oC	0000271	temperature-dependent growth	0000181	in vitro assay data	Decrease in RNA synthesis at high temperature. See figure a for 2B for gel electrophoresis results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: FB8 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbA+ Genotype of Experimental Strain : SDT1 sbaA-	Reference Condition:	0000252	metabolite accumulation	0000181	in vitro assay data	Decreased metabolism of ppGpp compared to the parent strain. See figure 3 a & b for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: FB8 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbA+ Genotype of Experimental Strain : SDT1 sbaA-	Reference Condition:	0000277	decreased beta-galactosidase activity	0000181	in vitro assay data	Significant decrease in beta-galactosidase activity compared to the parent strain. See Figure 4 for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: FB8 NCBI Taxon ID: 83333	Genotype of Reference Strain: sbA+ Genotype of Experimental Strain : SDT1 sbaA-	Reference Condition:	0000278	increased beta-galactosidase activity	0000181	in vitro assay data	the introduction of 1mM cAMP increased beta-galactosidase activity but less than the parent strain. See Figure 4 for the full experimental results.
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