PMID:6337120
| Citation |
Bogosian, G, Haydock, PV and Somerville, RL (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.J. Bacteriol. 153:1120-3 |
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| Abstract |
Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA. |
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| Keywords |
Bacterial Proteins; DNA, Bacterial; Escherichia coli; Indoles; Mutation; Operon; Repressor Proteins; Transcription, Genetic; Tryptophan; Tryptophan-tRNA Ligase |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay evidence |
Increase concentration of tryptophanyl-tRNA synthetase caused by the insertion of the second trpS gene caused increase resistance toward indolmycin. See Figure 2 for full experimental results. |
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay evidence |
the deletion of the trp repressor caused an increase resistance toward Indolmycin. See figure 2 for full experimental results. |
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</protect>
Notes
References
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