PMID:6365956
| Citation |
Trepeta, RW and Edberg, SC (1984) Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. J. Clin. Microbiol. 19:172-4 |
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| Abstract |
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and lactose fermentation is read directly from MacConkey agar. It was found that 4-methylumbelliferyl-beta-D-glucuronide could be incorporated directly into a modified MacConkey agar to directly detect the presence of beta-glucuronidase. Other characteristics of MacConkey agar were not affected. The incorporation of 4-methylumbelliferyl-beta-D-glucuronide into modified agar obviated the need for manufacture, quality control, and incubation of reagent-containing test tubes. The time needed to identify E. coli strains was reduced from 1 h to 5 min, and the ability to detect this species in mixed specimens was also enhanced. |
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| Keywords |
Agar; Culture Media; Escherichia coli/classification; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fermentation; Fluorescence; Glucuronates/metabolism; Glucuronidase/metabolism; Hymecromone/analogs & derivatives; Hymecromone/metabolism; Indoles/metabolism; Lactose/metabolism; Oxidoreductases/metabolism; Time Factors |
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Main Points of the Paper
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Materials and Methods Used
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- Methylumbelliferyl-β-D-glucuronide medium (FMG)
- Trypticase Soy Agar
- MacConkey Agar
- Microbial specimens obtained from Clinical Microbiology Laboratory, Yale-New Haven Hospital
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
References
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