PMID:6392473

Citation	Bruce, I, Hardy, J and Stacey, KA (1984) Potentiation by purines of the growth-inhibitory effects of sulphonamides on Escherichia coli K12 and the location of the gene which mediates this effect.J. Gen. Microbiol. 130:2489-95
Abstract	The increased toxicity of sulphonamides for Escherichia coli in the presence of low concentrations (50-100 microM) of purines or purine nucleosides has been confirmed and investigated further. The potentiating effect of a purine was dependent upon the activity of the appropriate phosphoribosyl transferase: a gpt mutant strain was not potentiated by guanine but remained fully sensitive to the addition of adenine. Mutants resistant to the potentiating effect of all purines have been isolated and partially characterized. The site of these mutations has been located in the region between oriC and asnA at minute 83 on the E. coli chromosome map. It is suggested that this locus be temporarily designated psp (potentiation of sulphonamides by purines) because these mutants have unaltered sensitivities to sulphonamides acting alone. Mutations in PurA, purR and folB did not affect the potentiation of sulphonamides by purines. Hypoxanthine-insensitive strains harbouring lambda asn20 were as sensitive as the wild-type to the potentiating effect. This result suggests that these lysogens are heterozygous for psp and that the wild-type allele is dominant. It is probable that psp is a regulatory gene, affecting some rate-limiting step in the biosynthesis of methionine.
Links	PubMed
Keywords	Chromosome Mapping; Drug Synergism; Escherichia coli; Genes, Bacterial; Mutation; Purines; Sulfanilamides; Sulfonamides; Vitamin B 12
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Strain IB3 NCBI Taxon ID: 83333	Genotype of Reference Strain: pabA Genotype of Experimental Strain : pabA	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to Sulfonamide
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Strain IB3 NCBI Taxon ID: 83333	Genotype of Reference Strain: pabB Genotype of Experimental Strain : pabB	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to Sulfonamide
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Strain IB3 NCBI Taxon ID: 83333	Genotype of Reference Strain: pabC Genotype of Experimental Strain : pabC	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to Sulfonamide
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Strain IB3 NCBI Taxon ID: 83333	Genotype of Reference Strain: pabD Genotype of Experimental Strain : pabD	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to Sulfonamide
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