PMID:6426767

Citation	Calcott, PH and Calcott, KN (1984) Involvement of outer membrane proteins in freeze--thaw resistance of Escherichia coli. Can. J. Microbiol. 30:339-44
Abstract	Two families of Escherichia coli mutants with altered outer membrane protein components were examined for sensitivity to freezing and thawing and other stresses. A mutant unable to make the lipoprotein (lpo) was extremely sensitive to freezing and thawing in water or saline and to challenge with detergent, while the mutant unable to make the porin proteins (ompB) was more resistant than the isogenic wild type; strains unable to make the tsx and ompA proteins were slightly more sensitive to the stresses. Similarly, the lpo deficient strain exhibited more and the ompB less wall and membrane damage than the wild-type strains. Little difference in the extent of wall damage, but more membrane damage, was seen for the two tsx and the ompA strains when compared with the wild-type strain. The roles of the specific proteins in determining sensitivity to freeze-thaw are discussed.
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : P400 tsx 200	Reference Condition:			ECO:0000181	in vivo assay evidence	Decreased resistance toward freeze thaw. This was also seen in Strain JE5506 which also lacked the tsx protein See figure 1 for experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompA+ Genotype of Experimental Strain : P460 ompA-1	Reference Condition:			ECO:0000181	in vivo assay evidence	Decreased resistance toward freeze-thaw. See Figure 1 for experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompB+ Genotype of Experimental Strain : ompB-105	Reference Condition:					Increase resistance toward freeze-thaw in both water and saline.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ lpo+ Genotype of Experimental Strain : tsx- lpo(del)	Reference Condition:					Significantly increased sensitivity toward freeze thaw. See Figure 1 for full experimental data.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompA+ Genotype of Experimental Strain : ompA-	Reference Condition:					Increased sensitivity toward Sodium lauryl sulfate.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx- in strain P407	Reference Condition:					Increased sensitivity toward Sodium lauryl sulfate. Similar results in strain JE5506.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompB+ Genotype of Experimental Strain : ompB- Strain P1578	Reference Condition:					Increased sensitivity toward sodium lauryl sulfate.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ lpo+ Genotype of Experimental Strain : tsx- lpo(del)	Reference Condition:					Significantly increased sensitivity toward sodium lauryl sulfate.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ Genotype of Experimental Strain : tsx-	Reference Condition:					Slight increase in resistance toward sodium chloride. See table 2.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: P400 NCBI Taxon ID: 83333	Genotype of Reference Strain: tsx+ lpo+ Genotype of Experimental Strain : tsx- lpo(del)	Reference Condition:					Slight increase in resistance toward sodium chloride. See table 2.
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References

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