PMID:6802799
| Citation |
Newman, EB, Malik, N and Walker, C (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.J. Bacteriol. 150:710-5 |
|---|---|
| Abstract |
Two methods for the direct isolation of spontaneous ssd mutants of Escherichia coli K-12 strains are described; (i) by growth with L-serine as the carbon source, and (ii) by low-level kanamycin resistance. A newly isolated mutant had the same phenotype as the mutant described previously, including inefficient use of the glucose, inability to growth with succinate, altered transport characteristics, and altered resistance to certain growth effectors. Succinate-utilizing derivatives which appear to be intragenic are characterized in detail. The relation between the mutants isolated here and mutants which are thought to have impairment in a system of coupling respiratory energy to active transport (ecfB mutants) is discussed. |
| Links | |
| Keywords |
Biological Transport, Active; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Glucose; Kanamycin; Mutation; Serine; Succinates; Succinic Acid |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0000266 |
utilizes carbon-containing compound |
Carbon utilization of L-Serine. See table 1 |
|||
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0000266 |
utilizes carbon-containing compound |
Carbon utilization of L-Serine. See table 1 |
|||
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0000276 |
increased resistance to antimicrobial compound |
Resistance to Kanamycin |
|||
|
a mutation or genetic difference within a strain |
|
|
|
OMP:0000276 |
increased resistance to antimicrobial compound |
Resistance to Neomycin |
| ||
| edit table |
</protect>
Notes
- L-serine phenotype:
- ECOID: This is a solid medium assay in minimal medium.
- Nemycin/kanamycin: It is an aminoglcoside and term has been requested.
References
See Help:References for how to manage references in omp dev.
