PMID:6997074
| Citation |
Dabbs, ER, Poldermans, B, Bakker, H and van Knippenberg, PH (1980) Biochemical characterization of ribosomes of kasugamycin-dependent mutants of Escherichia coli.FEBS Lett. 117:164-6 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Aminoglycosides; Anti-Bacterial Agents; Escherichia coli; Genotype; Methylation; Mutation; Ribosomal Proteins; Ribosomes; Species Specificity; tRNA Methyltransferases |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
- Methlayse activity was tested with MV mutants and it was shown that the ksgA<sup+ strains had activity the ksgA- strains did not.
- ksgA- strains were found to have ribomsomes with 16 S RNA which could be methylated in vitro whereas ksgA+" strains had ribosomes which could not be methylated in vitro. See table 1.
- The effect of ksg on fMet-tRNAf binding to ribosomes and polypeptide synthesis was tested. It was found that over a range of antibiotic concentration no dependence or stimulation due to Ksg was found. See table 2.
References
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