PMID:6998971

Citation	Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system.J. Bacteriol. 144:7-11
Abstract	Neither methyl-alpha-maltoside nor 5-thiomaltose is utilized by Escherichia coli as a sole carbon source. Both are, however, effective competitive inhibitors of maltose transport into the bacterium (Km for maltose, 0.8 microM, Ki for methyl-alpha-maltoside, 5.5 microM; Ki for 5-thiomaltose, 0.2 microM). Both analogs are bound by the periplasmic maltose-binding protein. Methyl-alpha-[14C]maltoside and 5-[3H]thiomaltose were both accumulated inside E. coli. Methyl-alpha-maltoside was unchanged after accumulation, but 5-thiomaltose was converted to an unidentified compound that could exit from the bacterium. Both analogs were inhibitory to the growth of E. coli, but only when the bacteria were previously induced for the maltose transport system. The analogs are substrates for but poor inducers of the maltose transport system.
Links	PubMed
Keywords	Biological Transport; Carrier Proteins; Escherichia coli; Maltose; Membrane Proteins
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: malE Genotype of Experimental Strain : malE(del)	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound	ECO:0000033	traceable author statement	Thio(5)maltose increase resistance for selection for mutant maltose transport system.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: malF Genotype of Experimental Strain : malF(del)	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound	ECO:0000033	traceable author statement	Thio(5)maltose increase resistance for selection for mutant maltose transport system.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: malG Genotype of Experimental Strain : malG(del)	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound	ECO:0000033	traceable author statement	Thio(5)maltose increase resistance for selection for mutant maltose transport system.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: malK Genotype of Experimental Strain : malK(del)	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound	ECO:0000033	traceable author statement	Thio(5)maltose resistance.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: malT Genotype of Experimental Strain : malT(del)	Reference Condition:	OMP:0000284	loss of utilization of carbon-containing compound	ECO:0000033	traceable author statement	Thio(5)maltose resistance.
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