PMID:7009552

Citation	Platz, A and Sjöberg, BM (1980) Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.J. Bacteriol. 143:561-8
Abstract	Recombinant plasmids containing all or part of the genetic region of Escherichia coli coding for the two subunits of ribonucleoside diphosphate reductase (proteins B1 and B2) were constructed with the aid of the multicopy plasmid pBR322. Two of these plasmids (pPS1 and pPS2) appeared to carry both a regulator and the complete structural information for the enzyme and, after transformation of E. coli, directed a 10- to 20-fold overproduction of both proteins B1 and B2. The other plasmids (pPS101 and pPS201) carried structural information for only protein B2. Cells carrying pPS1 and pPS2 showed a 5- to 500-fold increased resistance against the drug hydroxyurea. This establishes that in E. coli the inhibition of deoxyribonucleic acid synthesis by hydroxyurea is fully explained by its action on ribonucleotide reductase.
Links	PubMed
Keywords	DNA, Recombinant; Enzyme Induction; Escherichia coli; Genes; Hydroxyurea; Macromolecular Substances; Plasmids; Ribonucleoside Diphosphate Reductase; Ribonucleotide Reductases
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: KK535 NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdA Genotype of Experimental Strain : nrdA pPS1	Reference Condition:			ECO:0000039	protein assay data	increased resistance to hydroxyurea, figure 4B.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: KK535 NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdA Genotype of Experimental Strain : nrdA pPS2	Reference Condition:			ECO:0000039	protein assay data	resistance to hydroxyurea, figure 4B
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: KK535 NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdA Genotype of Experimental Strain : nrdA pPS101	Reference Condition:			ECO:0000039	protein assay data	slight increase in hydroxyurea resistance, figure 4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: KK535 NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdA Genotype of Experimental Strain : nrdA pPS201	Reference Condition:			ECO:0000039	protein assay data	slight increase resistance to hydroxyurea, figure 4.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdB Genotype of Experimental Strain : nrdB pPS1	Reference Condition:			ECO:0000039	protein assay data	increased resistance to hydroxyurea
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdB Genotype of Experimental Strain : nrdB pPS2	Reference Condition:			ECO:0000039	protein assay data	resistance to hydroxyurea, figure 4B
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdB Genotype of Experimental Strain : nrdB pPS101	Reference Condition:			ECO:0000039	protein assay data	slight increase in hydroxyurea resistance, figure 4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: nrdB Genotype of Experimental Strain : nrdB pPS201	Reference Condition:			ECO:0000039	protein assay data	slight increase resistance to hydroxyurea, figure 4
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