PMID:7028117
| Citation |
Slater, AC, Jones-Mortimer, MC and Kornberg, HL (1981) L-Sorbose phosphorylation in Escherichia coli K-12.Biochim. Biophys. Acta 646:365-7 |
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| Abstract |
L-Sorbose is phosphorylated by Escherichia coli by two distinct Enzymes II of the phosphoenolpyruvate-dependent phosphotransferase system. The glucose Enzyme II (specified by the gene ptsG) phosphorylates L-sorbose with an apparent Km of 0.08 +/- 0.03 mM and V of 31.8 +/- 3.5 nmol . mg-1 . min-1 whilst the fructose Enzyme II (specified by the gene ptsF) phosphorylates it with an apparent Km of 28.9 +/- 2.7 mM and V of 20.2 +/- 0.8 nmol . mg-1 . min-1. L-Sorbose induces neither of these Enzymes II, but sorbose inhibits the growth of strains expressing either of these functions constitutively. Mutants that have lost their sensitivity to L-sorbose are found to have lost either the glucose or the fructose phosphotransferase Enzyme II.U |
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| Keywords |
Escherichia coli; Genotype; Kinetics; Phenotype; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphotransferases (Nitrogenous Group Acceptor); Sorbose; Species Specificity |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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ECO:0000182 |
in vitro culture assay data |
Resistant to sorbose, table 1 |
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a mutation or genetic difference within a strain |
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ECO:0000182 |
in vitro culture assay data |
Mutant is resistant to sorbose, table 1 |
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Notes
Needs OMP term that does not exist yet.
References
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