PMID:7047497

Citation	Stewart, V and MacGregor, CH (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.J. Bacteriol. 151:788-99
Abstract	We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus.
Links	PubMed
Keywords	Apoenzymes; Chlorates; Coenzymes; Escherichia coli; Genes; Genes, Bacterial; Metalloproteins; Molybdenum; Mutation; Nitrate Reductases; Pteridines
edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5278 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-215 Genotype of Experimental Strain : nar-215::Tn10	Reference Condition:	OMP:0000298	enzyme activity phenotype	ECO:0000184	enzyme inhibition experiment data	mutant is narL - and has reduced Nitrate Reductase Activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5278 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-215 Genotype of Experimental Strain : nar-215::Tn10	Reference Condition:	OMP:0000298	enzyme activity phenotype	ECO:0000184	enzyme inhibition experiment data	mutant is narL- and has decreased formate activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5266 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-203 Genotype of Experimental Strain : nar-203::Tn10	Reference Condition:	OMP:0000298	enzyme activity phenotype	ECO:0000039	protein assay data	Mutant is NarK - and has an Incrase in nitrase reductase activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5266 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar203 Genotype of Experimental Strain : nar-203::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	Mutant is NarK - and has Reduced Formate activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5267 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-204 Genotype of Experimental Strain : nar-204::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	Mutant is NarI - and has reduced nitrate reductase activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5267 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-204 Genotype of Experimental Strain : nar-204::Tn10	Reference Condition:	ECO:0000039	protein assay data	OMP:0000269	protein activity phenotype	Mutant is NarI - and has reduced Formate activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5263 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-200 Genotype of Experimental Strain : nar-200::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	mutant is NarH - and there is reduced nitrate reductase activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5263 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-200 Genotype of Experimental Strain : nar-200::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	Mutant is NarH - and there is reduced formate activity.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5265 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-202 Genotype of Experimental Strain : nar-202::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	mutant is narG - and has reduced Nitrate reductase activity (roughly 1% of wildtype activity).
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5265 NCBI Taxon ID: 83333	Genotype of Reference Strain: nar-202 Genotype of Experimental Strain : nar-202::Tn10	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	Mutant is narG- and has Reduced Formate activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: fnr Genotype of Experimental Strain : fnr-	Reference Condition:	OMP:0000270	absence of growth	ECO:0000094	biological assay data	Mutants were sensitive to minimun 5 mM of chlorate.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5230-5233 NCBI Taxon ID: 83333	Genotype of Reference Strain: chlG Genotype of Experimental Strain : chlG::Mu cts	Reference Condition:	OMP:0000270	absence of growth	ECO:0000094	biological assay data	chlorate resistance
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: chlG Genotype of Experimental Strain : chlG::Mu cts	Reference Condition:	OMP:0000298	enzyme activity phenotype	ECO:0000039	protein assay data	decrease formate hydrogenlyase gas
a mutation or genetic difference within a strain	Taxon: Escherichai coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: chlG Genotype of Experimental Strain : chlG::Mu cts	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	reduced nitrate reductase activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: chlG Genotype of Experimental Strain : chlG::Mu cts	Reference Condition:	OMP:0000255	plating phenotype	ECO:0000039	protein assay data	deficient in the expression of tiary amine oxide reductase activity, as estimated by their phenotypes on MacConkey TMAO medium.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: clhE Genotype of Experimental Strain : chlE::mu cts	Reference Condition:	OMP:0000270	absence of growth	ECO:0000094	biological assay data	Chlorate Resistance
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255 NCBI Taxon ID: 83333	Genotype of Reference Strain: clhE Genotype of Experimental Strain : chlE::mu cts	Reference Condition:	OMP:0000269	protein activity phenotype	ECO:0000039	protein assay data	Inhibition of nitrate reductase activity
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255 NCBI Taxon ID: 83333	Genotype of Reference Strain: chlE Genotype of Experimental Strain : chlE::mu cts	Reference Condition:	ECO:0000039	protein assay data	OMP:0000269	protein activity phenotype	Inhibition of formate hydrogenlyase (formate dehydrogenase plus hydrogenase) thus failing to produce gas.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255 NCBI Taxon ID: 83333	Genotype of Reference Strain: chlE Genotype of Experimental Strain : chlE::mu cts	Reference Condition:	OMP:0000255	plating phenotype	ECO:0000039	protein assay data	Deficient in Tiary amine oxide reductase activity in anaerobic growth on MacConkey TMAO medium.
edit table

</protect>

Notes

The OMP term needs revision when appropriate OMP term is created and approved (in regards to enzyme activity).

References

See Help:References for how to manage references in omp dev.