PMID:765745

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Citation

Sprague, GF, Bell, RM and Cronan, JE (1975) A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport.Mol. Gen. Genet. 143:71-7

Abstract

An inorganic phosphate transport mutant has been isolated as a sn-glycerol-3-phosphate auxotroph and characterized genetically. Two lesions are responsible for the transport defect. One lesion, pst, is located at minute 74 of the E. coli genetic map while the other lesion, pit, is located at minute 68. All "K10" strains that were examined carry the pit lesion. Evidence is presented that the glycerol phosphate and hexose phosphate transport systems are not important inorganic phophate transport systems. The mapping data indicate that the genetic distance between malA and xyl is greater than that now allowed.

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Keywords

Bacterial Proteins; Biological Transport, Active; Chromosome Mapping; Chromosomes, Bacterial; Coliphages; Conjugation, Genetic; DNA, Bacterial; Escherichia coli; Genes; Glycerol-3-Phosphate O-Acyltransferase; Glycerolphosphate Dehydrogenase; Glycerophosphates; Mutation; Phenotype; Phosphates; Phospholipids; Phosphotransferases; RNA, Bacterial; Recombination, Genetic; Transduction, Genetic

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: 10B5
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: pstA
  • Genotype of Experimental Strain : pstA
  • Reference Condition:

Resistant to Arsenate

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: 10B5
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: pstB
  • Genotype of Experimental Strain : pstB
  • Reference Condition:

Resistant to Arsenate

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: 10B5
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: pstC
  • Genotype of Experimental Strain : pstC
  • Reference Condition:

Resistant to Arsenate

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: 10B5
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: pstS
  • Genotype of Experimental Strain : pstS
  • Reference Condition:

Resistant to Arsenate


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Notes

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