PMID:781181
| Citation |
Săsărman, A, Desrochers, M, Sonea, S, Sanderson, KE and Surdeanu, M (1976) Porphobilinogen-accumulating mutants of Salmonella typhimurium LT2.J. Gen. Microbiol. 94:359-66 |
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| Abstract |
Four independent porphobilinogen-accumulating mutants of Salmonella typhimurium LT2 were isolated by selecting for dwarf colony formation on neomycin agar media. Cell-free extracts of the parent strain, but not of the mutants, were able to convert 5-aminolaevulinic acid or porphobilinogen to porphyrins. The results indicated that the mutants were deficient in uroporphyrinogen I synthase (EC. 4.3.I. 8) activity: these are the first mutants of this type reported in S. typhimurium LT2. Mapping of the hemC locus (for uroporphyrinogen I synthase) by F-mediated conjugation and by P22-mediated transduction showed the gene sequence ilvEDAC-hemC-cya-metE. |
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| Keywords |
Aminolevulinic Acid; Catalase; Cell-Free System; Chromosome Mapping; Conjugation, Genetic; Genes; Hydroxymethylbilane Synthase; Mutation; Porphobilinogen; Porphyrins; Salmonella typhimurium; Transduction, Genetic |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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