PMID:8376351
| Citation |
Summers, WC and Raksin, P (1993) A method for selection of mutations at the tdk locus in Escherichia coli.J. Bacteriol. 175:6049-51 |
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| Abstract |
Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk. |
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| Keywords |
Drug Resistance, Microbial; Escherichia coli; Floxuridine; Genotype; Mutation; Thymidine Kinase |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000181 |
in vitro culture assay data |
Mutation at the tdk locus caused reduced thymidine kinase activity causing 5-flurodeoxyuridine resistance |
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Notes
- All 5-fluorodeoxyuridine resistant strain contained mutations that were mapped to a locus at 27.5 min.
References
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