PMID:8722757
| Citation |
Sandler, SJ, Samra, HS and Clark, AJ (1996) Differential suppression of priA2::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA, and dnaC. Genetics 143:5-13 |
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| Abstract |
First identified as an essential component of the phi X174 in vitro DNA replication system, PriA has ATPase, helicase, translocase, and primosome-assembly activities. priA1::kan strains of Escherichia coli are sensitive to UV irradiation, deficient in homologous recombination following transduction, and filamentous. priA2::kan strains have eightfold higher levels of uninduced SOS expression than wild type. We show that (1) priA1::kan strains have eightfold higher levels of uninduced SOS expression, (2) priA2::kan strains are UVS and Rec-, (3) lexA3 suppresses the high basal levels of SOS expression of a priA2::kan strain, and (4) plasmid-encoded priA300 (K230R), a mutant allele retaining only the primosome-assembly activity of priA+, restores both UVR and Rec+ phenotypes to a priA2::kan strain. Finally, we have isolated 17 independent UVR Rec+ revertants of priA2::kan strains that carry extragenic suppressors. All 17 map in the C-terminal half of the dnaC gene. DnaC loads the DnaB helicase onto DNA as a prelude for primosome assembly and DNA replication. We conclude that priA's primosome-assembly activity is essential for DNA repair and recombination and that the dnaC suppressor mutations allow these processes to occur in the absence of priA. |
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| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage phi X 174/genetics; Chromosomes, Bacterial; DNA Helicases/genetics; DNA Replication; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Dose-Response Relationship, Radiation; Escherichia coli/genetics; Escherichia coli/radiation effects; Escherichia coli Proteins; Genes, Bacterial/radiation effects; Genetic Markers; Mutagenesis; Phenotype; Recombination, Genetic; Replication Protein A; Repressor Proteins/genetics; Serine Endopeptidases/biosynthesis; Serine Endopeptidases/genetics; Suppression, Genetic; Transduction, Genetic; Ultraviolet Rays; beta-Galactosidase/biosynthesis |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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