PMID:9973363

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Citation

Dermić, D and Trgovcević, Z (1999) Specific effects of a recB mutation on the HfrH strain of Escherichia coli.J. Bacteriol. 181:1334-7

Abstract

The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer.

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Keywords

Conjugation, Genetic; Escherichia coli; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases; F Factor; Mutation

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: '3000HfrH'
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rec+HfrH
  • Genotype of Experimental Strain : recBHfrH
  • Reference Condition:

After the insertion of the Tn10 mutation the IRB101 cells grew extremely slowly. The generation time being four to five times longer than the parental strain's.


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