Difference between revisions of "PMID:1650909"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(Table edited by Wameza via TableEdit)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4032.D519bf449052e3-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="D519bf449052e3"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Itaya, M and Crouch, RJ'''  (1991) Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H. ''Mol. Gen. Genet.'' '''227''':433-7
 +
|-
 +
!align=left  |Abstract
 +
||
 +
The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1650909 PubMed]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.4032.D519bf449052e3&page=4032&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4032.D519bf449052e3-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.4032.K519bf4493803e-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K519bf4493803e"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: AB1157
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnhA<sup>+</sup>'' ''dnaA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnhA-1 dnaA508''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The mutation toward ''rnh'' enabled DnaA Independent origins to function. See table 2 for experimental data.
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: AB1157
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnhA<sup>+</sup>'' ''dnaA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnhA-59 dnaA508''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The mutation toward ''rnh'' enabled DnaA Independent origins to function. See table 2 for experimental data.
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: AB1157
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnhA<sup>+</sup>'' ''dnaA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnhA-91(amber) dnaA508''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The mutation toward ''rnh'' enabled DnaA Independent origins to function. See table 2 for experimental data.
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: AB1157
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnhA<sup>+</sup>'' ''dnaA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnhA-224 (UGA) dnaA508''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The mutation toward ''rnh'' enabled DnaA Independent origins to function. See table 2 for experimental data.
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: AB1157
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnhA<sup>+</sup>'' ''dnaA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnhA-339''::cat ''dnaA508''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 42<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The mutation toward ''rnh'' enabled DnaA Independent origins to function. See table 2 for experimental data.
 +
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: MIC1002
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 +
|
 +
*Genotype of Reference Strain: ''rnh<sup>+</sup>'' ''recB<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''rnh-1 recB270''
 +
|
 +
*Reference Condition: 32<sup>o</sup>C
 +
*Experimental Condition: 43<sup>o</sup>C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0000178
 +
|
 +
in vivo assay evidence
 +
|
 +
The ''rnh'' mutation caused a decrease in RNase H activity. See Table S3.
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.4032.K519bf4493803e&page=4032&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.4032.K519bf4493803e--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 12:14, 30 May 2013

Citation

Itaya, M and Crouch, RJ (1991) Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H. Mol. Gen. Genet. 227:433-7

Abstract

The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].

Links

PubMed

Keywords


edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: AB1157
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnhA+ dnaA+
  • Genotype of Experimental Strain : rnhA-1 dnaA508
  • Reference Condition: 32oC
  • Experimental Condition: 42oC

ECO:0000178

in vivo assay evidence

The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: AB1157
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnhA+ dnaA+
  • Genotype of Experimental Strain : rnhA-59 dnaA508
  • Reference Condition: 32oC
  • Experimental Condition: 42oC

ECO:0000178

in vivo assay evidence

The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: AB1157
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnhA+ dnaA+
  • Genotype of Experimental Strain : rnhA-91(amber) dnaA508
  • Reference Condition: 32oC
  • Experimental Condition: 42oC

ECO:0000178

in vivo assay evidence

The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: AB1157
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnhA+ dnaA+
  • Genotype of Experimental Strain : rnhA-224 (UGA) dnaA508
  • Reference Condition: 32oC
  • Experimental Condition: 42oC

ECO:0000178

in vivo assay evidence

The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: AB1157
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnhA+ dnaA+
  • Genotype of Experimental Strain : rnhA-339::cat dnaA508
  • Reference Condition: 32oC
  • Experimental Condition: 42oC

ECO:0000178

in vivo assay evidence

The mutation toward rnh enabled DnaA Independent origins to function. See table 2 for experimental data.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MIC1002
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rnh+ recB+
  • Genotype of Experimental Strain : rnh-1 recB270
  • Reference Condition: 32oC
  • Experimental Condition: 43oC

ECO:0000178

in vivo assay evidence

The rnh mutation caused a decrease in RNase H activity. See Table S3.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:1650909&oldid=10460"